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. 2021 Nov 27;12:100171. doi: 10.1016/j.mtbio.2021.100171

Fig. 8.

Fig. 8

Inhibition of miR-221-3p Reversed the Protective Effects of EVs on Apoptosis and Angiogenesis. (A) Expression values for miR-221–3p in MSCs-EVs and HIF-1α-EVs by qRT-PCR. (B) Experimental design. To inhibit miR-221–3p in HIF-1α-EVs. HIF-1α-EVs-inhibitormiR−221 and HIF-1α-EVs-inhibitorNC were incubated with cardiomyocytes or HUVECs under H/SD condition. (C and D) TUNEL staining at the border zone 4 weeks after MI, and quantification analysis of percentage of apoptotic cardiomyocytes between two groups. Scale bar ​= ​100 ​μm (E–F) Caspase3/caspase7 activity and quantification analysis in cardiomyocytes treated with HIF-1α-EVs-inhibitormiR−221 and HIF-1α-EVs-inhibitorNC under H/SD condition. Scale bar ​= ​100 ​μm (G) Representative images showing tube formation in HUVECs treated with HIF-1α-EVs-inhibitormiR−221 and HIF-1α-EVs-inhibitorNC. Scale bar ​= ​100 ​μm (H) Quantification of tube length in each group. Continuous variables and categorical variables were described by means ​± ​SEM and percentages. n=6 for each group. ∗P<0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001.