Fig. 9.

Moles of amino acid residues per mole of casein quantified by LC-MS before and after incubation of ubiquitin (panel A) or β-casein (panel B) with 100 mM AAPH for 90 min at 37 °C under 20% O₂ in phosphate buffer in the absence (PB) and presence of 60 mg mL⁻¹ dextran (D60). White bars correspond to control samples whilst light grey and dark cyan correspond to samples incubated with AAPH in the absence (PB) or presence (D60) of dextran. To minimize variations arising from protein hydrolysis, the data are normalized to the numbers of moles of Val quantified in each sample (as this residue is not target by ROO•) and then expressed as moles of residue per mole of protein. Data represent the means ± standard deviations of at least three independent experiments. Asterisks indicates data significantly (p < 0.05) different to the control samples. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)