Table 2.

Oligos used in this study, their functions, and their PCR conditions.

Oligo name	Function	Oligo sequence (5'--3')
USA1-F	PCR/Sequencing	CCTGGAGAAGATGTATGTGTT
USA1-R	PCR/Sequencing	TCTTCATGGTCGTGCTGAT
DUF1-F	PCR/Sequencing	TATCAAGTGTGTGGTGCC
DUF1-R	PCR/Sequencing	CGTGTTCGACAGATTGTCC
SLG1-F	PCR/Sequencing	GATGCTACTTATACTGGCGG
SLG1-R	PCR/Sequencing	TCAATCGGTTTCGTCTGG
HKR1-F	PCR/Sequencing	GAGAAAGCTATTGCTTTTGGT
HKR1-R	PCR/Sequencing	TCAATAGATGATGGCTGCAC
H09053-F	PCR/Sequencing	AATACGAAAGCCACGACG
H09053-R	PCR/Sequencing	ATATCTGGAATGCACTACCTG
A02255-F	PCR/Sequencing	TGGATTCGAATGAGGGACT
A02255-R	PCR/Sequencing	CGAGTTCACACCGATTATCC
G00825-Fex	PCR/Sequencing	TCAAATGCTCCTCCTGGC
C00825-F1	Sequencing	CTCTACAGGCGGCAAAA
G00825-R1	Sequencing	GAATAATTAGAGTCGCCTCCG
25F-N	Sequencing	TGG CAG AAA TAA ACG CCA G
G00825-Rex	PCR/Sequencing	CCAACATCAATTTCAGGAGC
SSR1-F	PCR/Sequencing	GAGCTGGAACTCGATCCG
SSR1-R	PCR/Sequencing	AGGAAGGGGAGTAATGATGG
PIR2-F	PCR/Sequencing	ATGCAATACAAAAAGACTCTAGC
PIR2-R	PCR/Sequencing	TGGATCATTTGGTGCCTTAC
PIR1-F	PCR/Sequencing	CTTCTTCCTCTGTCGCTAAG
PIR1-R	PCR/Sequencing	CAATTTGAGAAGCAGCGC
C00715-F	PCR/Sequencing	AGCCTCTGTCCCTACTTTATC
C00715-R	PCR/Sequencing	GTCGCTGTTGGTGTGTAA
G03839-F	PCR/Sequencing	CCCAATCCCTTTCTCTGCT
G03839-R	PCR/Sequencing	TATCCTCTTCTCATCGCTCG
G06281-F	PCR/Sequencing	GATGTTATGGTCTAGCTTTGC
G06281-R	PCR/Sequencing	CTGCCTATCTTAGATTGCTAGA

Note that except for G00825 and PIRI, the rest of the loci had the same PCR program (94 °C 5 mins, 35 cycles of (94 °C 30 s, 60 °C 30 s, 72 °C 40 s), 72 °C 8 mins). PCR programs for G00825 was 94 °C 5 mins, 35 cycles of (94 °C 30 s, 58 °C 30 s, 72 °C 2 mins), 72 °C 8 mins and for PIRI was 94 °C 5 mins, 35 cycles of (94 °C 30 s, 58 °C 30 s, 72 °C 40 s), 72 °C 8 mins.