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. 2021 Nov 18;57(96):12980–12983. doi: 10.1039/d1cc04612h

Fig. 1. Site-directed spin labelling of a membrane transporter in intact E. coli. (A) Schematic overview of the site-directed spin labelling procedure using the genetically encoded ncAA SCO-l-lysine and the PaNDA spin label. (B) Chemical structure of TEMPO-CNCbl. (C) Crystal structure of BtuB (PDB entry 1NQH) with PaNDA rotamers at loop site 404. The purple and light pink shade indicate the rotamer populations for the two possible regioisomers resulting from the conjugation of PaNDA to SCO-l-lysine, as determined by MtsslWizard. TEMPO-CNCbl with its rotamers determined with MMM is drawn in orange.

Fig. 1