Fig. 3. Temporal control of the paramagnetic potential of the PaNDA spin label in situ. E. coli expressing BtuB_F404 → SCO were spin labelled with PaNDA, mixed with TEMPO-CNCbl (which corresponds to the indicated timepoint 0 min), and cw EPR spectra were recorded (orange shades; spectra were averaged over 12 scans). When the TEMPO-CNCbl derived signal had vanished, cells were irradiated and cw EPR spectra were recorded to measure the PaNDA-derived signal (purple shades; spectra were averaged over 8 scans).
