Figure 5.

A multi-chamber system with inserted functionally graded scaffolds and different number of vascular channels to promote performing several experiments with a single device. (top) Device CAD design and laser stereolithography prototype of rapid mold for PDMS casting of the outer device frame. (bottom) Final PDMS frame, for needle placement, scaffold insertion or hydrogel casting and vasculature formation by needle removal, obtained by vacuum casting and placed upon microscope slide and fluorescently labeled endothelial cells (labeled with Calcein, green) lining the inside of a collagen channel in the device. Scale bar = 100 μm.