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A hydrogel-based complex vasculature, complete with bifurcation, varied vessel size, and wells for co-culture of a parenchymal cell type at varying distances from the vasculature to study biotransport effects. (left) PDMS mold for the device and (right) hydrogel device seeded with endothelial cells (green) in the vascular channels and breast cancer cells (red) in the adjacent wells. Scale bar = 3 mm.
