CHX arrests ribosomes in the PRE translocation state with the nascent peptide attached to the A-site tRNA. (A) A- and P-site tRNAs at the peptidyltransferase center, with the nascent peptide attached to the A-site tRNA CCA end as shown by the EM density. (B) A-site tRNA anticodon interaction with the mRNA A-site codon. (C) P-site tRNA anticodon interaction with the mRNA P-site codon. A-site tRNA, blue; P-site tRNA, green; mRNA, purple; nascent peptide, various colors; rRNA, gray; uL16, yellow; and uL3, orange.