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Time-resolved measurement of adaptor protein recruitment to GRPR. Cells were stimulated with different concentrations of the reference compound AMBA at the indicated time point (dashed line). (A) BRET-based assay for miniGq recruitment. Nanoluciferase complementation assay for β-arrestin-1 (B), β-arrestin-2 (C), GRK2 (D), GRK3 (E), and GRK5 (F). Measurements for all other compounds are shown in SI Appendix, Fig. 4.
