Fig. 6.

Consequences for biased signaling. Pairwise comparison of recruitment assays. The reference compound AMBA (black) was considered as nonbiased or neutral. (A) The dimer-inducing compounds (AG-2-BBN-2/-3) show higher-Gq efficacy than the reference compound, while the β-arrestin efficacy does not defer from the reference compound. (B) The nondimer–inducing bivalent compound (AG-2-BBN-1) shows a strong bias toward Gq signaling, while the dimer-inducing compounds show a bias toward β-arrestin signaling. (C) The dimer-inducing compounds showed different β-arrestin potency bias. (D) Schematic summary of obtained results.