Figure 6.

The nucleolin targeting aptamer, AS1411, inhibits SW1343, RD and SW872 cell viability and migration. A. Pearson’s correlation between nucleolin (NCL) mRNA expression levels and CRISPR-derived gene dependency score of NCL across 850 cancer cell lines (CCL) (green) and 24 sarcoma cell lines (blue). B. Cells were treated with 10 μM AS1411 or CRO for 96 h after which an MTT assay was performed. Two-way ANOVA was used to compare groups, data are the mean ± SEM of three independent experiments performed in quadruplicate, *P<0.05; **P<0.01; ***P<0.001. C. IC₅₀ values for AS1411-treated SW1353, SW872 and RD cells. D. Cells were treated with 10 μM AS1411 or CRO for 96 h after which 2D-Scratch motility assays were performed. Bar graphs show the distance migrated between 3-9 h. Results are representative of three independent experiments. Student’s t-test was used to compare between groups, **P<0.01; ***P<0.001; error bars represent mean ± SEM.