Figure 2.

^CD7ΔCD7-CAR-T cells are fratricide-resistant after CD7 blockade and CD7 blockade was specific targeting to CD7. A. Schematic of the CD7 blockade structure. B. Data illustrate the ratio of CD7 blockade or CAR⁺ in activated T cells by flow cytometry. CAR was detected using a goat anti-human IgG Fc antibody. C. Fold expansion of T cells and CD7-CAR-T cells transduced with or without CD7 blockade and measured at different time points by cell counting. Data represent three independent experiments (n=3 donors each). D. Flow cytometric analysis showing the expression of CD7 in blank and CD7 blockade Jurkat cells. E. The standard s-shaped curve shows different binding ratios of blank and CD7 blockade Jurkat cells after incubation with the humanized anti-CD7 nanobody under serially-diluted concentrations for 1 h, followed by incubation with the Alexa Flour 647-labeled anti-human Fc antibody.