Figure 6.
CD7ΔCD7-CAR-T cells exert anti-leukemic activity in a CCRF-CEM CDX model. A. B-NDG mice were injected i.v. with 0.2×105, 1×105, or 5×105 Luc+-GFP+-CCRF CEM cells, respectively (n=3), with 1×105 CCRF-CEM cells/mouse used as a control. The median survival of the mice was 19, 20, and 25 days, respectively. B. Flow cytometric analysis of the tissue distribution of tumor cells (CD45+GFP+) in mice. CCRF-CEM cells were mainly distributed in BM, lung, and liver, and a small number of tumor cells infiltrated the spleen and peripheral blood. BM, bone marrow. C. Schematic of the experiment. B-NDG mice were infused i.v. with 3×106 CD7-CAR-T cells or control 2 days after a single i.v. injection of 1×105 Luc+-GFP+-CCRF CEM cells. Mice injected with PBS were used as controls. D. The survival of mice are analyzed by Mantel-Cox log-rank test and shown with Kaplan-Meier curves and **P<0.01. E. Statistical analysis of platelet counts (PLT) in different treatment groups. CONT, healthy mice.