Figure 2.

miR-155 up-regulation in THP-1 cells co-cultured with HCV⁺/⁻ Huh7.5 cells. THP-1 cells were co-cultured with HCV⁺ or HCV⁻ Huh7.5 cells, followed by real-time RT-PCR analysis of miR-155 expression. (A) Huh7.5 cells were transfected with JFH-1 or control (HCV⁻ Huh7.5). HCV core protein (red) was detected by immunofluorescence staining after 48 h, and Hoechst was used to visualize the nuclei. Scale bar represents 50 µm. (B) HCV RNA from the culture supernatant was detected 24 h after transfection. (C) Total RNA was isolated after 24 h of transfection and subjected to TaqMan miRNA assay for miR-155.