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. 2021 Nov;9(21):1618. doi: 10.21037/atm-21-2620

Figure 3.

Figure 3

miR-155 induces TNF-α production and activates JNK signaling during chronic HCV infection. CD14+ monocytes isolated from chronically HCV-infected individuals were transfected with miR-155 inhibitors or negative control while CD14+ monocytes isolated from HS were transfected with miR-155 mimics or negative control for 24 h. TNF-α and JNK1, 2, 3 were detected by ELISA and RT-PCR. (A) Transfection efficiency was measured by flow cytometry after 6 h transfection of CD14+ monocytes with fluorescent (FAM) miR-155 mimics and fluorescent (FAM) miR-155 inhibitors. (B) TNF-α secreted in the culture media was measured by ELISA. (C) After CD14+ monocytes were transfected with miR-155 mimics or miR-155 inhibitor, the expression of JNK1 (n=6 or 12), JNK2 (n=9 or 6), and JNK3 (n=9 or 6) was determined by RT-PCR. (D) HCV core and NS5 antigens promote the activation of JNK signaling, including JNK and p65 in THP-1 cells.