Figure 1.

Analysis of the DNA methylation dynamics during zebrafish germ cell development reveals a unique reprogramming pattern

A. The schematic diagram for zebrafish germ cell developmental stages in this study. Two transgenic strains were used to track the development of germ cells in zebrafish. Dotted boxes indicate the major developmental processes of germ cells at different stages. B. Global DNA methylation levels during zebrafish germ cell development. The methylation level was calculated as the average across all covered CpGs in the genome. DNA methylation data of zebrafish sperm, oocyte, and MBT stages were obtained from GEO: GSE44075. C. A dendrogram showing the clustering of DNA methylomes during zebrafish germ cell development. The R package ‘ape’ was used to perform hierarchical clustering analysis with ‘euclidean’ distance and the ‘complete’ clustering method (500 bp bin for each unit). D. A brief summary of expression patterns of key genes and key biological processes during zebrafish germ cell development. PGC, primordial germ cell; hpf, hours post fertilization; dpf, days post fertilization; dF, days post fertilization female; dM, days post fertilization male; FACS, fluorescence-activated cell sorting; WGBS, whole genome bisulfite sequencing; MBT, midblastula transition.