Figure 3.

The tet3 mutation can cause the reduction of PGC numbers in zebrafish juveniles

A. Histogram of the gene expression levels of tet1/tet2/tet3 in PGCs. Gene expression level was averaged from two biological replicates. B. Schematic diagram showing CRISPR/Cas9 editing in zebrafish tet3 gene, with an 8 bp deletion in exon 3. C. Whole mount in situ hybridization showing expression of vasa mRNA in WT and tet3^c1 mutant fish at the 5 dpf stage. D. Number of PGCs at 5 dpf counted under stereoscopic microscope (WT, n = 43; tet3^c1, n = 27). The data are represented by mean ± SD. P values are calculated by t-test. **, P < 0.01. WT, wild-type.