Figure 4.

Aromasin treatment can induce female-to-male sex transitionin adult zebrafish

A. The schematic diagram for zebrafish SR samples in this study. vasa::egfp transgenic zebrafish was used to induce sex transition by aromasin treatment. The samples include WT ovary, gonads from zebrafish treated with aromasin for 1 month, 2 months, or 3 months, SR testis, and WT testis. B. Observation of gonad morphological change in control female and aromasin-treated fish. Left, gonads under bright filed; middle, GFP fluorescence; right, H&E staining. Red boxes indicate the regions of weak fluorescence of testis. The scale bar in H&E staining indicates 100 µm. C. Time-course clustering analysis of gene expression patterns throughout zebrafish SR process. For the four distinct expression clusters, the left plots of each cluster show the gene expression pattern, the middle tables show the GO enrichment results, and the right plots show dynamics of representative gene expression levels. SR, sex-reversed; H&E, Hematoxylin and Eosin.