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. 2021 Nov 22;118(48):e2104459118. doi: 10.1073/pnas.2104459118

Fig. 6.

Fig. 6.

Ccp1 associates with centromeres during mitosis in the cnp20-14A mutant. (A) Schematic representation of the WT CIM domain and its phospho-null mutants. (B) GFP-Ccp1 associates with centromeres during mitosis in the cnp20-14A mutant. Cnp20- or Cnp20-14A-mCherry was used as a centromere marker. (Scale bar, 2 μm.) (C) Quantification of the percentage of indicated cells showing centromeric localization of GFP-Ccp1 in mitosis (M) and G2. Experiments were performed in triplicate. At least 40 cells were scored in one single experiment. Error bars represent mean and SD. *P < 0.05. ns, no significant differences. (D) TBZ sensitivity of phospho-null or phosphomimetic CIM mutants. Cells grew on the rich YES medium in the absence or presence (15 μg/mL) of TBZ at 30 °C for 3 to 5 d. (E) Time-lapse microscopy of Spc25-GFP and Sad1-mCherry in WT (Top) and cnp20-14A (Bottom). Time was measured from the separation of SPB (0 min). Sad1-mCherry was used as an SPB marker. (Scale bar, 1 μm.) (F) Quantification of the percentage of indicated cells that failed to complete chromosome segregation in 30 min. Experiments were performed in triplicate. At least 40 cells were scored in one single experiment. Error bars represent mean and SD. *P < 0.05.