. 2021 Nov 5;32(12):2766–2776. doi: 10.1021/jasms.1c00206

Table 2. Comparison of Conditional Cu(II) Binding Constants Obtained with Competition Experiments with Histidine of the Studied Peptides at pH = 7.4 (^CK_7.4), Compared to These Constants Determined Previously by Other Methods and Published^a.

peptide sequence (name)	published log ^CK_7.4	log K_d from His competition ESI-MS experiments in this study	log ratio log K_d (ESI-MS) - log ^CK_7.4 (published)	range of concentrations of histidine in this study (μM)
DTHFPI-NH₂ (hepc6)	14.66^b	13.98	–0.68	1000-10
FRHDSGYEVHHQK-NH₂ (Aβ4–16)	13.53^c	12.84	–0.69	500-1

Logarithmic values are given for better clarity. The published log ^CK_7.4 values were determined by potentiometry and corroborated by spectroscopic methods.

Reference (63).

Reference (6).

Table 2. Comparison of Conditional Cu(II) Binding Constants Obtained with Competition Experiments with Histidine of the Studied Peptides at pH = 7.4 (CK7.4), Compared to These Constants Determined Previously by Other Methods and Publisheda.

Table 2. Comparison of Conditional Cu(II) Binding Constants Obtained with Competition Experiments with Histidine of the Studied Peptides at pH = 7.4 (^CK_7.4), Compared to These Constants Determined Previously by Other Methods and Published^a.