TABLE 2.
Kinetics of extension from nucleotides opposite m6G by yeast Polη
| DNA substratea | Km (μM) | Vmax (%/min) | Vmax/Km | foext |
|---|---|---|---|---|
| Extension from C or T opposite G | ||||
| 5′----CATC | 0.42 ± 0.02 | 5.3 ± 0.11 | 12.6 | 1.0 |
| -----GTAGAA-- | ||||
| 5′----CATT | 24.7 ± 4 | 1.58 ± 0.37 | 0.063 | 5 × 10−3 |
| -----GTAGAA-- | ||||
| Extension from G, A, T, or C opposite m6G | ||||
| 5′----CATG | 7.6 ± 2.1 | 1.69 ± 0.13 | 0.22 | 1.7 × 10−2 |
| -----GTAGAA-- | ||||
| | | ||||
| m6 | ||||
| 5′----CATA | 13.4 ± 1.2 | 1.24 ± 0.08 | 0.093 | 7.4 × 10−3 |
| -----GTAGAA-- | ||||
| | | ||||
| m6 | ||||
| 5′----CATT | 11.47 ± 0.25 | 3.6 ± 0.1 | 2.45 | 1.9 × 10−1 |
| -----GTAGAA-- | ||||
| | | ||||
| m6 | ||||
| 5′----CATC | 0.81 ± 0.12 | 4.8 ± 0.13 | 5.93 | 4.7 × 10−1 |
| -----GTAGAA-- | ||||
| | | ||||
| m6 |
a
dTTP was added to each reaction mixture.