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. 2021 Nov 26;13(11):1797–1812. doi: 10.4252/wjsc.v13.i11.1797

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©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/

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The influence of mitogen-activated protein kinase kinase 7 knockdown on the apoptosis of human spermatogonial stem cells. A-C: Flow cytometry and fluorescein isothiocyanate (FITC) Annexin V analysis of the percentages of early (A and B) and late (A and C) apoptosis cells in human spermatogonial stem cells (SSCs) transfected with the Control-small interfering RNA (siRNA) and mitogen-activated protein kinase kinase 7 (MKK7)siRNA 3; D and E: Terminal deoxynucleotidyl transferase nick-end-labeling (TUNEL) assays of the percentages of TUNEL⁺ cells in human spermatogonial stem cells transfected with the Control-siRNA and MKK7-siRNA 3. Scale bars in D: 50 μm. ^aP < 0.05 denotes a significant difference between the MKK7-siRNA 3 and Control-siRNA groups. dUTP: Deoxyuridine 5’-triphosphate; DAPI: 4,6-diamidino-2-phenylindole.