Skip to main content
. 2021 Dec;2(12):e695–e703. doi: 10.1016/S2666-5247(21)00195-6

Figure 2.

Figure 2

Tissue tropism and intracellular localisation of Klebsiella pneumoniae in mouse spleens following intravenous infection

(A) Quantitative distribution of K pneumoniae between CD169+, F4/80+, MARCO+ macrophages, and the white pulp in the mouse spleen. Data are representative of two entire tissue sections of approximately 1 × 2 cm per mouse. (B) Size of bacterial clusters associated with host cells at 6 h after infection. Statistical significance was determined by one-way ANOVA. 30 random fields of view were analysed with 60 × magnification from the Olympus confocal microscope. Symbols represent individual bacterial clusters. (C) 3D reconstruction of a K1 focus of infection Z-stack acquired by confocal microscopy showing the top of the cell layer, and a single cut into the cell. DAPI stain indicated in blue, CD169 in red, and K1 ST23 in green. (D) K1 and K2 co-infected tissue section stained with purified IgG raised against K1 (green) capsule, or K2 (red). Lower magnification co-localisation images for all serotypes, in addition to confocal Z-stacks are shown in the appendix (pp 10–11). DAPI=4′,6-diamidino-2-phenylindole. hvKp=hypervirulent K pneumoniae.