Table 1.
Identification of elenolic acid (EA) and derivatives in isenolic® by high performance liquid chromatography-associated Mass Spectrometry (HPLC-MS).
| Compound | TR (min) | λmax (nm) | [M-H]− (m/z) | Fragments (m/z) | 2[M-H]− (m/z) |
|---|---|---|---|---|---|
| Demethyl EA glucoside (DMEA-Glu-1) (Oleoside) | 20.6 | 233 | 389.1 | 227.0, 183.0 | 779.2 |
| Demethyl EA glucoside (DMEA-Glu-2) (Secologanoside) | 34.0 | 236 | 389.2 | 345.1, 138.9 | ND |
| Caffeoyl diglucoside+ EA glucoside 1 (EA-Glu 1) | 37.9 | 231/300sh/312 | 489.0 403.0 | 463.0 ([M + Cl]-), 241.1, 139.1 | ND |
| EA glucoside 2 (EA-Glu 2) | 40.6 | 240/342 | 403.0 | 223.1, 179.0, 119.1, 112.9, 100.9 | 807.3 |
| Demethyl EA glucoside (DMEA-Glu-3) | 47.0 | 238 | 389.0 | 139.1 | ND |
| 6-hydroxyloganin+ EA glucoside 3 (EA-Glu 3) | 56.6 | 246 | 405.1 403.1 | 240.9 | 811.0 |
| EA 1 (EA-1) | 62.7 | 239 | 241.1 | 165.1, 139.1, 127.1, 121.1, 111.2, 101.0 | ND |
| EA 2 (EA-2) | 66.7 | 240 | 241.1 | ND | ND |
| EA derivative | 73.7 | 281 | 395.0 | 241.1, 209.1, 181.1, 139.1 | ND |
Retention time (Rt), UV-Vis absorption maximum (λmax) and characteristic molecular and fragments ions. ND: not detected.