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Quantitative real-time PCR (qRT-PCR) validation of selected unigenes and miRNAs. A qRT-PCR was performed to determine the expression levels of 11 unigenes relative to actin. B The expression of 6 known miRNAs and 2 novel miRNAs was verified by stem-loop qRT-PCR with 18S RNA as an internal reference gene. Error bars indicate ±SD (n = 3, from three technical replicates). * indicates a significant difference at p < 0.05 compared with CK using a two-tailed Student’s t-test
