FIG. 1.

53BP2 levels increase following UV irradiation-induced DNA damage. (A) Western blots of lysates (30 μg of total protein per lane) prepared from GM38 cells (left panels) and HT1080 cells (right panels) 24 h after UV irradiation with 0, 5, or 10 J/m². The fold increase of 53BP2 protein levels compared to no-UV-irradiation levels are indicated below, normalized for tubulin. 53BP2 was detected with monoclonal antibody DX547; p53 was detected with monoclonal antibody 1801. (B) Northern blot of total RNA (15 μg/lane) prepared from HT1080 cells 24 h after UV irradiation at the indicated doses, probed for 53BP2, stripped, and reprobed for actin. The fold increase of 53BP2 mRNA is indicated below, normalized for actin.