FIG. 7.

Attenuation of 53BP2 induction enhances clonogenic survival following UV irradiation. (A) Surviving fraction of HT1080 cells after introduction of AS-oligo (■) or control-oligo (⋄). Oligonucleotide-treated cells were incubated for 5 h, followed by counting, replating, and UV irradiation at the indicated doses. Shown is a representative experiment with the means and standard deviations from triplicate plates. (B) Western blot using anti-53BP2 monoclonal antibody DX547 of equivalent amounts of lysates (50 μg of total protein per lane) prepared from HT1080 cells after the introduction of AS-oligo or control-oligo. Oligonucleotide treated cells were incubated and UV irradiated in parallel with the clonogenic assay. Lysates were prepared 24 h after UV irradiation. Anti-hsc70 monoclonal antibody was used to confirm equal loading.