Fig. 3.

The AIM2 inflammasome is activated in AD patients and APP/PS1 transgenic mice. A–D Brain transcriptome data from patients with AD and controls were collected from the GEO database and normalized for analysis. E–H APP/PS1 transgenic mice at the age of 9 months were anesthetized and euthanized to obtain the cerebral cortex and hippocampus. E Detection of the expression levels of AIM2, ASC, pro-caspase-1, caspase-1 and IL-1β in the cerebral cortex by western blotting. β-actin served as the internal control. In the right panel, ImageJ software was used to semiquantitatively analyze the western blotting results. F qRT-PCR was used to detect the mRNA expression of AIM2 and ASC in the cerebral cortex. GAPDH served as internal control. G The expression of AIM2, ASC, pro-caspase-1, caspase-1 and IL-1β in the hippocampus was detected by western blotting. β-actin served as the internal control. H The mRNA expression of AIM2 and ASC in the hippocampus was detected by qRT-PCR. GAPDH was used as the internal control. The data present means ± S.E. of independent experiment. APP/PS1 transgenic mice were compared with WT mice *P < 0.05, **P < 0.01, ***P < 0.001