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. 2021 Oct 14;218(12):e20211327. doi: 10.1084/jem.20211327

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© 2021 Lu et al.

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Figure 4. — Antigen recognition and clonal expansion of public clonotype 5 (TCR-017/018). (A) TCRβ-017, -018, or mutants at position 94 were coexpressed with TCRα-017/018 and human CD4 in reporter cells. Cells were kept unstimulated or stimulated with peptide S_864–882 or anti-CD3 antibody in the presence of APCs from donor Ts-018. Percentages of GFP⁺ cells in the CD3⁺ populations are shown. Data are shown as mean ± SD of duplicates. (B and C) Crystal structure of TCRαβ heterodimer of clonotype 5 (TCR-017). The entire view (B) and a close-up of the CDR3 domains (C) are shown. Q94 and adjacent amino acids, C90, A91, S92, and S93 within CDR3β are indicated. (D) Occurrences of extended antigen-reactive TCRβ chains harboring the 17 acceptable substitutions at position 94 (shown as X; except for D94 of TCRβ-017/018) as determined in A within individuals in a prepandemic healthy cohort from Japan (n = 27) and within cohorts of prepandemic healthy donors (n = 786) and convalescent COVID-19 patients (n = 1,413) from multiple ethnicities. Numbers of individuals possessing this TCRβ among cohorts are indicated. (E) Frequencies of extended antigen-reactive TCRβ chains harboring the 17 acceptable substitutions at position 94 (except for D94 of TCRβ-017/018) as determined in A within the prepandemic healthy donors, all convalescent COVID-19 patients, and patients admitted to the ICU and non-ICU setting from multiple ethnicities. Horizontal line, median; bottom/top, first/third quartile; whiskers, 1.5 times the interquartile range. Data are representative of two independent experiments (A). *, P < 0.05; ***, P < 0.00001.