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. 2021 Oct 8;218(12):e20211003. doi: 10.1084/jem.20211003

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© 2021 Shinnakasu et al.

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Figure 4. — Immunization with CoV-2 GM antigens leads to the activation of CoV-1 and CoV-2 cross-reactive GC B cells. (A) Representative flow cytometry plots analyzing GC B cells (CD138⁻B220⁺IgD⁻IgM⁻GL7⁺Fas⁺) from draining lymph nodes (dLNs) of mice 3 wk after primary immunization with each antigen. The plots are representative of findings from two independent experiments (left). Quantification of absolute numbers of GC B cells (right) from multiple mice in one experiment. Results are representative of two independent experiments. CoV-2 RBD WT (n = 5); GM9 (n = 5); GM14 (n = 5). (B) Representative flow cytometry plots analyzing CoV-2 RBD–binding dLN GC B cells (CD138⁻B220⁺IgD⁻IgM⁻GL7⁺Fas⁺CoV-2 RBD⁺). Plots are representative of findings from two independent experiments (left). Quantification of absolute numbers of GC B cells (right) from multiple mice in one experiment. Results are representative of two independent experiments. CoV-2 RBD WT (n = 6); GM9 (n = 6); GM14 (n = 6). (C) Cross-reactivity of CoV-2 RBD-binding dLN GC B cells (CD138⁻B220⁺IgD⁻IgM⁻GL7⁺Fas⁺CoV-2 RBD [APC]⁺ CoV-2 RBD [PE-Cy7]⁺) with CoV-1 RBD assessed by flow cytometry. Plots are representative of findings from two independent experiments (left). The frequency of CoV-1 and CoV-2 RBD cross-reactive GC B cells (right) from multiple mice in one experiment. Results are representative of two independent experiments. CoV-2 RBD WT (n = 6); GM9 (n = 6); GM14 (n = 6). Horizontal lines indicate mean values; each symbol indicates one mouse. *, P < 0.05; **, P < 0.01; unpaired Student’s t test.