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. 2021 Oct 8;218(12):e20211003. doi: 10.1084/jem.20211003

Figure S1.

Figure S1.

Design and expression of antigens, and effective induction of anti–CoV-2 RBD antibodies after immunization with nanoparticle antigen. (A) RBD-ACE2 complex. Non-conserved residues between CoV-1 and CoV-2 are colored in white. (B) The parental amino acid sequences and introduced NXT sequons (left). SDS-PAGE of RBD WT, GM9, and GM14 (right). (C) ELISA plots for CoV-2 RBD WT probe recognition of sera from respective biotin (+) RBD/Streptavidin nanoparticles, biotin (−) RBD/Streptavidin nanoparticles, or only biotin (+) RBD-immunized mice 3 wk after primary immunization or preimmune mice. The CR3022 mouse IgG1 mAb (Invivogen) was used as a standard. Representative of two independent experiments. Horizontal lines indicate mean values. Biotin (+) RBD/Streptavidin nanoparticles (n = 5); biotin (−) RBD/Streptavidin nanoparticles (n = 5); only biotin (+) RBD (n = 5); preimmune sera (n = 3). Dotted lines indicate detection limit. Horizontal lines indicate mean values; each symbol indicates one mouse. *, P < 0.05; **, P < 0.01; unpaired Student’s test. Ag, antigen.