Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 8;218(12):e20211003. doi: 10.1084/jem.20211003

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Shinnakasu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S4. — Reactivity and phylogenetic analysis of mAbs. (A) Correlation between gMFI from bead-based flow-cytometric assay and Rmax from biolayer interferometry (Octet) assay. gMFI and dissociation constant [KD(M)], maximal R (RMax), association rate (kon; 1/ms), and dissociation rate (koff) of bivalent mAb-CoV-2 RBD interactions. Spearman coefficient and P value were calculated by Prism software. (B) The phylogenetic tree of clonal lineages of GC-derived clones from GM14-1 and GM14-2 mice. The root is unmutated germline ancestor. The blue circles are inferred sequences. The white and red colors of the circles indicate the intensity of CoV-2 RBD binding in bead-based flow-cytometric assay (Table S1). The circles with stars are clones with high neutralization activity against CoV-2 (Fig. 5 C).