Table 3.
LoD of single malarial infections calculated for the RealStar® Malaria PCR and the RealStar Malaria Screen&Type PCR assays
| Real-time PCR Kit | Species | LoD sample 1 | LoD sample 2 | LoD sample 3 | Mean + StD |
|---|---|---|---|---|---|
| RealStar genus | P. falciparum | 0.5 p/µL | 0.07 p/µL | – | 0.28 ± 0.09 p/µL |
| RealStar species | P. falciparum | 0.70 p/µL | 0.14 p/µL | – | 0.42 ± 0.15 p/µL |
| P. ovale | 0.58 p/µL | 2.00 p/µL | 1.20 p/µL | 1.60 ± 1.01 p/µL | |
| P. vivax | 0.09 p/µL | 0.27 p/µL | – | 0.18 ± 0.01 p/µL | |
| P. malariae | 0.23 p/µL | 0.46 p/µL | – | 0.34 ± 0.02 p/µL | |
| P. knowlesi | 0.30 p/µL | 0.20 p/µL | – | 0.25 ± 0.005 p/µL |
LoD = limit of detection; RealStar-genus = RealStar Malaria PCR; RealStar-species = RealStar Malaria Screen&Type PCR; p/µL = parasites/µL; StD = standard deviation.