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. 2021 Oct 14;220(12):e202106130. doi: 10.1083/jcb.202106130

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© 2021 Sarangapani et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S4. — Control experiments related to Fig. 5. (A) Kinetochore material purified by immunoprecipitation of Dsn1-His-Flag from cells carrying epitope-tagged WT Ndc80-3HA (SBY19186), phospho-deficient Ndc80-8A-3HA (SBY19855), or phospho-mimetic Ndc80-8D-3HA (SBY19877), visualized by silver stain after SDS-PAGE before mass spectrometry analysis. (B) Results from mass spectrometry analysis of the same material visualized in A. Similar numbers of peptide spectrum matches were detected for the indicated kinetochore proteins across each of the three samples.