Figure 5.

OIP5-AS1 acts as a ceRNA for miR-186 in GC cells. A – Bioinformatical predication of interaction between OIP5-AS1 and miR-186. B – Dual luciferase reporter assay showed that miR-186 mimics reduced the luciferase activity of OIP5-AS1-WT in AGS and SGC-7901 cells. C – The expression levels of miR-186 in AGS and SGC-7901 cells after transfection were examined by RT-qPCR analysis. *p < 0.05 vs. sh-NC-transfected cells. D – The expression levels of miR-186 in GC tissues and adjacent nontumour tissues were examined by RT-qPCR analysis. E – The expression levels of miR-186 in five GC cell lines and normal human gastric mucosa cells GES-1 were examined by RT-qPCR analysis. *p < 0.05 vs. GES-1 cells. F – Pearson correlation analysis between OIP5-AS1 and miR-186 expression levels in GC tissues