Figure 1.

The CPE (50 μg/mL) treatment increased in lipid clearance and had no effect on cell viability of HepG2 cells. (A) Representative image of oil red O (ORO)-stained HepG2 cells, which were treated with 0.5 mM OA and PA combination in a 2:1 ratio for 16 h, followed by 24 h post-treatment with 50 μg/mL of CPE, compared to lipid mixture-treated cells and control cells (no lipid was added) (n = 3). (B) Changes in intracellular lipid content were assessed by measuring ORO absorbance at 520 nm. **p < 0.01 vs. no lipid treated cells, #p < 0.05 vs. lipid mixture-treated cells. (C) Cell viability was determined by the CCK-8 assay. Values are expressed as percentage of the control. Data represent mean ± SDs of three independent experiments. *p < 0.05 vs. every other group.