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. 2020 Sep 2;58(1):838–844. doi: 10.1080/13880209.2020.1803365

Figure 4.

Figure 4.

Role of autolysosomes in CPE-mediated clearance of intracellular lipid in HepG2 cells. (A) HepG2 cells treated with 50 μg/mL of CPE or 1 μM of rapamycin (RAPA) for 24 h after 0.5 mM of OA + PA mixture treatment for 16 h, followed by double labelling with Lyso-tracker red (red) and Bodipy493/503 (green) showed increased colocalization of autolysosomes and cellular lipids (yellow dots) vs. OA and PA treated cells and no lipid-treated cells. (B) Electron micrograph images of HepG2 cells treated with 50 μg/mL of CPE or 1 μM of RAPA for 24 h after 0.5 mM of OA + PA mixture treatment for 16 h showed autolysosomes filled with lipids vs. OA and PA treated cells and no lipid-treated cells. Arrow indicated the lipid droplets and autolysosome structure was denoted with arrow heads. RAPA was used as a positive control.