Figure 3.
CA IX increases in the lung tissue but not in the plasma or other major organ tissue of rats with pneumonia. P. aeruginosa (1 × 107 cfu in 200 μl of PBS) was introduced into the tracheas of Fischer rats. Twenty-four hours later, after ultrasound was completed, blood samples were drawn from the Abd aorta, and lung, kidney, heart, brain, liver, and diaphragm tissues were harvested. (A) Western blot analysis showed no change in plasma CA IX concentrations with pneumonia, which is consistent with human data. CA IX was significantly increased in (B) lung tissue but not in (C–G) other organs examined in pneumonia rats. Data represent the mean ± SD. A Student’s t test was used to compare different groups. *Significant difference (P < 0.05) from control. Readers may view the uncut gels for B, D, E, and G in the data supplement.