Figure 6.

P. aeruginosa infection increases CA IX expression and release in PMVECs. (A and B) CA IX mutant PMVEC cell lines were seeded at 5.0 × 10⁵ cells/well on 6-well plates on bicarbonate-buffered media. Two days after cell seeding, media were changed to HBSS, and cells were treated with P. aeruginosa at a multiplicity of infection of 0.055 and with DMSO (0.1%) or marimastat (10μM). Six hours later, the CA IX protein abundance was measured in cell lysates and supernatants by using Western blotting. P. aeruginosa infection significantly increased CA IX protein expression and release, and marimastat (MM) reduced extracellular ectodomain cleavage. At least five separate experiments were performed. One-way ANOVA and Bonferroni post hoc tests were used to compare different groups. *Significant difference (P < 0.05) from DMSO control. ^†Significant difference (P < 0.05) from DMSO infection. Readers may view the uncut gels for B in the data supplement. ns = not significant.