Figure 5.

Inhibition of HDAC6 prevents CSE-induced collagen synthesis and BSMC and PASMC proliferation. (A–D) Human BSMCs (A and B) and human PASMCs (C and D) were incubated with CSE (1%) in the absence and presence of the HDAC6 inhibitor Tub A (1μM) for 24 hours, after which the collagen-I protein levels (A and C) and cell proliferation (B and D) were measured. (E–H) Human BSMCs (E and F) and human PASMCs (G and H) were transfected with siRNAs against HDAC6 or Cntr siRNA, respectively. After 72 hours, the cells were incubated with CSE (1%) for 24 hours, and then the protein levels of collagen-I and HDAC6 (in E and G) and the level of cell proliferation (in F and H) were measured. In A, C, E, and G, the blots are representative immunoblots of four independent experiments. In B, D, F, and H, bar graphs depict changes in cell proliferation. Results are expressed as means ± SEs; n = 4. *P < 0.05 versus Cntr and ^#P < 0.05 versus CSE. Cont =  control.