Figure 3.

JNK(c-Jun N-terminal kinase)/c-Jun–mediated Wnt11 induction of myofibroblast differentiation. Normal HLFs treated with Wnt11 at the indicated doses were analyzed for total and phosphorylated (A) JNK and (B) c-Jun by using Western blotting. The quantified protein level of p-JNK or p-c-Jun was expressed as the percentage of total JNK or c-Jun after normalization to their respective GAPDH signals. Normal HLFs were also treated with 400 ng/ml Wnt11 in the presence of 10μM sp600125 (JNK inhibitor) and were then analyzed for total and phosphorylated (C) c-Jun as well as (D) α-SMA by using Western blotting. GAPDH signals were used as loading Conts for all the Western blots. Representative blots are shown. N = 4 in A and N = 3 in B–D. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.