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. 2021 Mar 30;65(5):473–488. doi: 10.1165/rcmb.2021-0152TR

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Figure 3. — Directed differentiation protocols designed to produce EPCs. (A) Schematic of stepwise directed differentiation designed to generate EPCs from embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs). (B) Directed differentiation of human iPSCs (hiPSCs) into cord blood ECFCs (Prasain and colleagues, 2014). (C) Differentiation of hiPSCs to CD34⁺CD31⁺ EPCs (Lian and colleagues, 2014). BMP = bone morphogenetic protein; EGM-2 = endothelial growth medium-2; FGF = fibroblast growth factor; HPP = high proliferative potential; VEGF = vascular endothelial growth factor.