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. 2021 Dec 3;17(12):e1010098. doi: 10.1371/journal.ppat.1010098

Fig 5. GNB1 protein bound more strongly to H9N2 virus-derived M1 protein than to H5N1-derived M1 protein.

Fig 5

(A–D) Interaction between influenza M1 and endogenous GNB1 proteins was determined by PLA. 293T cells were transfected with (A) H5N1-derived M1 plasmid, (B) H9N2-derived M1 plasmid, (C) H3N2-derived M1 plasmid, or (D) empty vector as negative control. The PLA was performed using antibodies specific to influenza M1 and endogenous GNB1 proteins. The fluorescence of cells was analyzed by a fluorescence confocal microscope (red fluorescent signal). Nuclei were stained with DAPI (blue). (E) Multiple images (A–D) were processed by BlobFinder to measure the PLA fluorescence intensity per cell (~30 cells total for each condition). Graphs show means ± SD of three independent experiments (*, P < 0.05; **, P < 0.01).