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. 2021 Dec 3;17(12):e1010098. doi: 10.1371/journal.ppat.1010098

Fig 9. GNB1 protein contributed to viral particle release triggered by H9N2 virus-derived M1 protein.

Fig 9

(A–E) Sizing and molecular profiling of VLP-associated H9N2-derived eGFP-M1 in #297 siRNA-induced GNB1-silenced or normal 293T cells. (A) A549 cells were transfected with siRNAs (targeting GNB1 or negative control), and GNB1 protein expression level at 36 h post-transfection were determined by Western blotting. (B–C) Size profiling of H9N2-derived eGFP-M1+ VLP particles with the FITC channel. (D–E) Molecular profiling of H9N2-derived eGFP-M1+ VLP particles. SS-H, side scatter height. (F–J) GNB1 knockdown decreased rM14:M-H9N2 virus replication in A549 cells. (F) GNB1 protein expression level at 36 h post-transfection determined by Western blotting. A549 cells transfected with siRNAs (either NC or GNB1-targeting siRNA #297) were infected with (G) rM14:M-H5N1 virus or (H) rM14:M-H9N2 virus at an MOI of 1. Viral titers were measured by TCID50 assay at the indicated time points. Each data is represented as means ± SD and represents three independent experiments. (**, P < 0.01; ***, P < 0.001; ns, no significance). A549 cells transfected with NC or GNB1-targeting siRNA #297 were infected with (I) rM14:M-H5N1 virus or(J) rM14:M-H9N2 virus at an MOI of 1, and expression of HA and M1 protein was measured by Western blotting.