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. 2021 Dec 3;7(49):eabj8156. doi: 10.1126/sciadv.abj8156

Fig. 4. MON-2 is required for longevity by regulating trafficking between the Golgi complex and endosomes.

Fig. 4.

(A to C) Microscopic images of transgenic animals expressing mon-2::gfp. The mon-2::gfp transgene was expressed in neurons (arrows) (A), the intestine (B), and neurons and seam cells (an arrow and arrowheads, respectively) (C). odr-1p::rfp was used as a coinjection marker (arrowhead) in (A). Scale bars, 20 μm. (D) Fluorescence microscope images of an intestinal cell in a transgenic animal expressing mon-2::gfp, rfp::rab-10 and the overlay of the two images. Scale bar, 10 μm. (E and F) Colocalization of mammalian GFP-MON2 with giantin, a Golgi marker, was decreased under starvation conditions (E), whereas that with RAB11, a recycling endosome marker, was increased (F) in HeLa cells. Scale bars, 10 μm. (G) Quantification of (E) and (F) (N = 8, 8, 12, and 12, respectively). Error bars represent SEMs (*P < 0.05 and **P < 0.01, two-tailed Student’s t test). Each dot represents a result from a single cell. (H to J) pad-1 RNAi (H), snx-3 RNAi (I), or tbc-1 RNAi (J) reduced the longevity of isp-1(qm150) [isp-1(−)] mutants. See table S5 for specific values and statistical analyses of the life-span data shown in this figure.