Fig. 2. C1qbp ablation in T cells leads to defective antiviral effector CD8⁺ T cell response.

(A) Flow cytometry of total splenocytes (top row) and splenic 2CD4⁺ (middle row) and splenic CD8⁺ (bottom row) T cells from WT and C1qbp-tKO mice at day 8 after infection with the LCMV Armstrong strain. (B) Frequency and total number of splenic CD4⁺ or CD8⁺ (among splenocytes), CD44^hiCD62L^lo effector CD4⁺ or CD8⁺ T cells (among splenic CD4⁺ or CD8⁺ T cells) from mice as in (A) (n = 6 per group). (C) Flow cytometry of splenic CD8⁺ T cells from mice as in (A). (D) Frequency (among splenic CD8⁺ T cells) and total number of NP_396–404 (NP396)–specific (top row) and GP_33–41 (GP33)–specific (bottom row) CD44⁺ CD8⁺ cells from mice as in (A) (n = 6 per group). (E) Flow cytometry of splenic NP396-specific (top row) and GP33-specific (bottom row) CD44⁺ CD8⁺ T cells from mice as in (A). (F) Frequency [among NP396-specific (top row) or GP33-specific (bottom row) CD44⁺ CD8⁺ T cells] and total number of NP396- or GP33-specific KLRG1⁺ effector CD8⁺ T cells in spleens of mice as in (A) (n = 6 per group). (G) Flow cytometry of splenic CD8⁺ T cells from mice as in (A). (H) Frequency (among splenic CD8⁺ T cells) and total number of IFN-γ⁺ (top row) or TNF-𝛂⁺ (bottom row) CD44⁺ CD8⁺ T cells in spleens of mice as in (A) (n = 6 per group). Each symbol represents an individual mouse; small horizontal lines indicate the means (±SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; ns, not significant (two-tailed unpaired Student’s t test). Data are representative of three independent experiments (error bars, SD).