| Cell line (human) |
SV-589 |
PMID:6091915
|
RRID: CVCL_RW34
|
SV40 transformed human fibroblasts |
| Cell line (human) |
SV-589/PDP1-Myc-FLAG |
This paper |
N/A |
SV-589 cells stably expressing tetracycline-inducible human PDP1-Myc-FLAG |
| Antibody |
Anti-SREBP-2 (rabbit polyclonal) |
PMID:25896350
|
IgG-22D5 |
(1–5 µg/ml) |
| Antibody |
Anti-UBIAD1 (mouse monoclonal) |
PMID:29167270
|
IgG-1H12 |
(1–5 µg/ml) |
| Antibody |
Anti-HMGCR (mouse monoclonal) |
PMID:22143767
|
IgG-A9 |
(1–5 µg/ml) |
| Antibody |
Anti-PDP1 (goat polyclonal) |
Santa Cruz Biotechnology |
Cat#SC-163253; RRID:AB_10842717
|
(1–5 µg/ml) |
| Antibody |
Anti-RhoA (mouse monoclonal) |
Santa Cruz Biotechnology |
IgG-26C4; Cat#SC-418; RRID:AB_628218
|
(1–5 µg/ml) |
| Antibody |
Anti-Rap1 (mouse monoclonal) |
Santa Cruz Biotechnology |
IgG-E6; Cat#SC-398755 |
(1–5 µg/ml) |
| Antibody |
Anti-Cdc42 (mouse monoclonal) |
Santa Cruz Biotechnology |
IgG-B8; Cat#SC-8401; RRID:AB_627233
|
(1–5 µg/ml) |
| Antibody |
Anti-Calnexin (rabbit polyclonal) |
Novus Biologicals |
Cat#NB100-1965; RRID: AB_10002123
|
(1–5 µg/ml) |
| Antibody |
Anti-LSD-1 (rabbit polyclonal) |
Cell Signaling Technology |
Cat#2139; RRID: AB_2070135
|
(1–5 µg/ml) |
| Antibody |
Anti-T7•Tag Antibody (mouse monoclonal) |
Sigma Millipore |
IgG2b; Cat#69522; RRID:AB_11211744
|
(1–5 µg/ml) |
| Antibody |
Anti-FLAG M2 (mouse monoclonal) |
Sigma-Aldrich |
IgG1; Cat#3165; RRID:AB_262044
|
(1–5 µg/ml) |
| Transfected construct (human) |
pCMV/TO-PDP1-Myc-FLAG |
This paper |
Cat#V102020 |
Human PDP1-Myc inserted into pCDNA4/TO vector (Thermo Fisher) |
| Transfected construct (hamster) |
pCMV-HMGCR (TM1-8)-T7 |
PMID:12535518
|
|
Membrane domain of hamster HMG CoA reductase inserted into pcDNA3 |
| Transfected construct (human) |
siRNA against PDP1 (PDP1 siRNA-A)GGCGCGAGGUGCUGAUGAAUU |
Dharmacon/Thermo Fisher Scientific |
|
|
| Transfected construct (human) |
siRNA against PDP1 (PDP1 siRNA-B)GGCGCGAGGUGCUGAUGAAUU |
Dharmacon/Thermo Fisher Scientific |
ON-TARGET plus |
|
| Transfected construct (human) |
siRNA against PDP1 (PDP1 siRNA-C)CGACGUAGCUUUUGGCUUUUU |
Dharmacon/Thermo Fisher Scientific |
|
|
| Transfected construct (human) |
siRNA against PDP1 (PDP1 siRNA-D)AGUACAGCAUCGUGGACUAUU |
Dharmacon/Thermo Fisher Scientific |
|
|
| Transfected construct (human) |
siRNA against PDP1 (SMARTpool)(PDP1 siRNA-E)GCACAAUGUCACCGACGUA ACAUAAGCUUUCUCGUAUA GUAAACUGAACUUCGAGAA GCCCUGAUGUCGAGGUUA |
Dharmacon/Thermo Fisher Scientific |
ON-TARGETplus Human PLPP6 (403313) siRNA |
|
| Transfected construct (jellyfish) |
siRNA against GFPCAGCCACAACGUCUAUAUCUU |
PMID:24025715
|
|
|
| Commercial assay or kit |
Lipofectamine RNAiMAX |
Thermo Fisher Scientific |
Cat#13778500 |
|
| Commercial assay or kit |
X-tremeGENE HP DNA Transfection Reagent |
Roche Diagnostics |
Cat#45274000 |
|
| Commercial assay or kit |
Click-IT Protein Reaction Buffer Kit |
Thermo Fisher Scientific |
Cat#c10276 |
|
| Commercial assay or kit |
Novex WedgeWell 4%–20%, Tris-Glycine, 1.0 mm, Mini Protein Gel, 15-well |
Thermo Fisher Scientific |
Cat#c10276 |
|
| Chemical compound, drug |
Cholesterol |
Sigma-Aldrich |
Cat#C8667 |
|
| Chemical compound, drug |
Azido-geranylgeraniol |
Thermo Fisher Scientific |
Cat#C10249
|
|
| Chemical compound, drug |
Geranylgeraniol |
Sigma-Aldrich |
Cat#G3278 |
|
| Chemical compound, drug |
Menaquinone-4 |
Sigma-Aldrich |
Cat#809,896 |
|
| Chemical compound, drug |
Benzonase Nuclease |
Sigma-Aldrich |
Cat#E1014-5KU |
|
| Chemical compound, drug |
25-Hydroxycholesterol |
Avanti Polar Lipids |
Cat#700019 P |
|
| Chemical compound, drug |
Trans, trans-farnesol |
Sigma-Aldrich |
Cat# 277,541 |
|
| Chemical compound, drug |
GGTI-298 |
Cal Biochem |
Cat# 345,883 |
|
| Chemical compound, drug |
MG-132 |
Peptides |
Cat# 12 L-3175-V |
|
| Software, algorithm |
Image J (Fiji) |
NIH |
|
|
