Figure 4.

SIRT1 may be a hypothetical direct miR-141 target. (a) Luciferase report contains the miR-141 targeting sequences and mutated version of the SIRT1 3′ UTR wild type, including the site where the binding agent was altered (red). The luciferase reporter gene containing wild-type (Wt) or mutant SIRT1 3′ UTR (Mut) was cotransfected into SH-SY5Y cells by simulating NC or miR-141. (b) Luciferase activity was measured and normalized 72 h after transfection. (c) The protein level of transfected NC or miR-141 mimics and SNHG15-siRNA cells was detected by western blotting.