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. 2021 Oct 4;20:15330338211045213. doi: 10.1177/15330338211045213

Figure 6.

Figure 6.

Effect of 5-FU on the TRPM2-modulated TE-1 cell proliferation and apoptosis. (A) Summary data showing that 5-fluorouracil (5-FU, 5 μM; 24-h incubation) suppresses TE-1 cell proliferation and showing the effect of a TRPM2 channel inhibitor (ACA, 20 μM) and TRPM2 siRNA (siTRPM2). Cell proliferation is detected by a CCK-8 assay and expressed as OD. Data are shown as the mean ± SEM; n = 5. *P < .05 versus control (Ctl); #P < .05 versus 0 μM ACA; &P < .05 versus siRNA control (siNC); P > .05 versus Ctl. (B) Summary data showing the effect of 5-FU (5 μM, incubation for 24 h) on the protein expression level of cleaved caspase-3 and the effect of ACA and siTRPM2. β-tubulin is used as a loading control. Data are shown as the mean ± SEM; n = 6. *P < .05 versus Ctl; #P < .05 versus 5-FU; &P < .05 versus siNC + 5-FU.

Abbreviations: TRPM2, transient receptor potential cation channel subfamily M, member 2; ACA, anthranilic acid; 5-FU, 5-fluorouracil; TE-1 cells, cell line; CCK-8, Cell Counting Kit-8; OD, optical density.