FIG. 1.

Ftz(Q50K) fails to activate transcription from natural enhancer elements in Drosophila S2 cells. (A) Transient-transfection assays. Two reporter genes, hb-CAT and kni-CAT, were used in transient-transfection assays to determine the activity of HA-tagged Bcd and Ftz(Q50K). Shown are CAT assay results from cells cotransfected with effector plasmids bearing the gene encoding either no protein (lanes 1 and 4), Bcd (lanes 2 and 5), or Ftz(Q50K) (lanes 3 and 6). (B) Western blot analysis. Antibodies against HA were used in the Western blot assay. Lanes 1 to 3 represent results using Drosophila cell lysates containing no activator, Bcd, and Ftz(Q50K), respectively.